A colorimetric assay of pancreatic lipase: Rapid detection of lipase and colipase separated by gel filtration
- 1 January 1985
- Vol. 20 (1) , 42-45
- https://doi.org/10.1007/bf02534361
Abstract
A rapid assay for pancreatic lipase (E.C., glycerol-ester hydrolase 3.1.1.3) is described. The assay is based on the color change of a pH indicator as butyric acid is released from the substrate tributyrin. A mixture made with tributyrin and the water soluble components of the assay is ideally suited for use as a rapid test as, for example, when assaying chromatography fractions. Quantitative data can be obtained by measuring the disappearance of absorbance at 557 nm versus a blank reaction. The assay has been used in the rapid preparation of colipase-free lipase and colipase.Keywords
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