Differential tolerance of hydroxylamine by an Alcaligenes sp., a heterotrophic nitrifier, and by Nitrobacter agilis

Abstract
An Alcaligenes sp. isolated from soil and shown to be an active heterotrophic nitrifier was found to be highly tolerant of hydroxylamine. Growth of this organism occurred in medium containing as much as 235 mg NH2OH-N∙L−1. Joint culturing of the Alcaligenes sp. and Nitrobacter agilis in medium containing 18 mg NH2OH-N∙L−1 resulted in the eventual synthesis of nitrite and nitrate. However, N. agilis cultured separately in the presence of hydroxylamine at concentrations as low as 5 mg NH2OH-N∙L−1 was subsequently unable to convert nitrite to nitrate.

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