Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acids
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (12) , 4891-4904
- https://doi.org/10.1093/nar/5.12.4891
Abstract
The non-covalent binding of ethidium monoazide to nucleic acids is entirely analogous to that of ethidium (binding constant approximately 2-3 X 10(5) M). The ethidium monoazide can be photochemically covalently linked to nucleic acids in high yield, up to 75%, by long wavelength light. The fluorescence of ethidium monoazide and ethidium crosslinked to nucleic acids show the same environmental sensitivity as does the fluorescence of ethidium. These properties of ethidium monoazide indicate its use as a fluorescent photoaffinity label for nucleic acids. Ethidium diazide can be photochemically linked to nucleic acids but appears to have properties substantially different from those of ethidium.Keywords
This publication has 21 references indexed in Scilit:
- Photobinding of 8-methoxypsoralen to transfer RNA and 5-fluorouracil-enriched transfer RNABiochemistry, 1978
- Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNABiochemistry, 1977
- Actinomycin D complexes with oligonucleotides as models for the binding of the drug to DNA. Paramagnetic induced relaxation experiments on drug-nucleic acid complexesBiochemistry, 1977
- High resolution proton nuclear magnetic resonance investigation of the structural and dynamic properties of d(C15A15)·d(T15G15)Biochemistry, 1977
- Production of frameshift mutations in salmonella by a light sensitive azide analog of ethidiumMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1976
- Selective covalent binding of an ethidium analog to mitochondrial DNA with production of petite mutants in yeast by photoaffinity labelingJournal of Molecular Biology, 1975
- Use of photochemically induced crosslinking as a conformational probe of the tertiary structure of certain regions in transfer ribonucleic acidBiochemistry, 1973
- Kinetics of ethidium bromide as a probe of transfer ribonucleic acid structureBiochemistry, 1973
- The change of the torsion of the DNA helix caused by intercalation: II — Measurement of the relative change of torsion induced by various intercalating drugsBiochimie, 1971
- Studies of the binding of ethidium bromide to transfer ribonucleic acid: Absorption, fluorescence, ultracentrifugation and kinetic investigationsJournal of Molecular Biology, 1969