Biological activities of chemically synthesized N‐acetylneuraminic acid‐(α2→6) ‐monosaccharide analogs of lipid A

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Abstract
The mitogenicity and lethal toxicity of chemically synthesized lipid A analogs, in which 2,3‐acyloxyacylglucosamine‐4‐phosphate linked to tetraacetyl‐N‐acetylneuraminic acid (compound A‐207) or to N‐acetylneuraminic acid (compound A‐307), were examined. Although the mitogenic activity of the synthetic compounds was weaker than that of bacterial LPS, doses of 10–50 μg/ml of A‐207 and 5–10 μg/ml of A‐307 were capable of increasing incorporation of [3H]thymidine into cultured spleen cells of C57BL/6 mice. Lethal toxicity of A‐207 was observed at 10 μg/mouse in C57BL/6 mice sensitized with D‐galactosamine hydrochloride. However, the attachment of tetraacetyl‐N‐acetylneuraminic acid or N‐acetylneuraminic acid does not appear to enhance the biological activity of acyloxyacylglucosamine‐4‐phosphate.