Phytochrome and Seed Germination. V. Changes of Phytochrome Content During the Germination of Cucumber Seeds

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Abstract
Cucumber seeds are light-sensitive, dark-germinating seeds. Inhibition of germination can be induced by prolonged exposure to continuous or intermittent far-red radiation (FR). The dark germination process and the response to FR are phytochrome controlled. Phytochrome can be detected in these seeds by differential spectrophotometry in vivo. Spectrophotometrically measurable phytochrome increases during dark germination. The rate of increase is temperature dependent. Light treatments which are inhibitory for germination result in phytochrome contents lower than those of the seeds germinating in darkness. Treatments which restore germination also restore phytochrome formation.