Nature of Albumin Exported from the Frog Oocyte Following Microinjection of Mouse Liver mRNA
- 1 March 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 91 (3) , 933-938
- https://doi.org/10.1093/oxfordjournals.jbchem.a133782
Abstract
Microinjection of mouse liver mRNA into Xenopus laevis oocytes induced efficient export of a polypeptide with an apparent Mr of 68,000 which was immunoprecipitable with anti-mouse albumin antibody. Analysis of the anti-albumin precipitate of the exported protein by two-dimensional gel electrophoresis showed that the electrophoretic behavior precisely coincided with that of authentic mouse serum albumin. This result indicates that the Xenopus oocyte may perform secretory processes similar or identical to those occurring in liver cells with respect to the processing of albumin.Keywords
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