ACTIVATED RAT NEUTROPHILS - CORRELATION OF ARACHIDONATE PRODUCTS WITH ENZYME-SECRETION BUT NOT WITH O-2-. GENERATION

  • 1 January 1985
    • journal article
    • research article
    • Vol. 120  (1) , 112-120
Abstract
Functional responses (enzyme secretion and generation of O2- were studied in rat neutrophils with the use of a variety of different agonists which vary in their ability to activate neutrophils. Concomitantly, the cells were analyzed for production of cyclooxygenase (PGE2, PGE2.alpha. and TXB2 [thromboxane B2]) and lipoxygenase (mono-HETE [prostaglandin E2] and LTB4) products in order to determine whether there is a correlation between functional responses and arachidonate products in stimulated neutrophils. The studies indicate that enzyme secretion is closely correlated with generation of arachidonate (cyclooxygenase as well as lipoxygenase) products. These responses are dependent on the dose of agonist employed, but there does not appear to be a unique pattern of arachidonate products that can be attributed to a specific agonist. With respect to monohydroxyeicosatetraenoic aid (monoHETE) generation, it was demonstrate that stimulated rat neutrophils selectively produce 5-HETE, to the virtual exclusion of 12-HETE and 15-HETE. The production of O2- from stimulated rat neutrophils is correlated neither with enzyme secretion nor with the generation of arachidonate products. Enzyme secretion and O2- generation apparently are mediated by 2 fundamentally different intracellular pathways, even though these functional responses probably derive from common agonist-receptor interactions on the plasma membrane.

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