Multiple restriction fragment length polymorphism genotypes of Porphyromonas gingivalis in single periodontal pockets
- 1 April 1995
- journal article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 10 (2) , 125-128
- https://doi.org/10.1111/j.1399-302x.1995.tb00132.x
Abstract
A total of 188 Porphyromonas gingivalis strains isolated from 13 periodontal pockets of 8 periodontitis patients were investigated by means of restriction fragment length polymorphism (RFLP) analysis using the fimA gene as a pobe. A total of 5 RFLP genotypes were identified, and over half of the isolates belonged to type I. Four of the 8 patients harbored only 1 RFLP genotype of P. gingivalis, and 1 patient harbored only 2 RFLP genotypes in different sites. On the other hand, in 3 other patients, multiple RFLP genotypes were found in single periodontal pockets. Further studies will be required to clarify whether multiple genotypes of P. gingivalis colonized a single periodontal pocket simultaneously or whether a mutation occurred in the fimbrilin gene locus of P. gingivalis.Keywords
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