Binding of Protein A to Some Human Gamma-Globulins Used Intravenously

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Abstract
The binding capacity was examined of radioiodinated protein A ([125I] PA) for several human IgG. Most of them are commercial preparations used in immunosubstitution therapy. Two experimental approaches were chosen. In the 1st, the binding of [125I] PA to solidified IgG preparations was studied. In the 2nd approach, the inhibition of [125I] PA binding to a given Ig by various human Ig samples was established. Both the binding and inhibition criteria were used to estimate the order of the relative binding capacities of the Fc region in studied IgG preparations. The various commercial .gamma.-globulin preparations were compared using single radial immunodiffusion and cross-immunoelectrophoretic techniques.