Production and Purification of Thermostable Cellulases fromHumicola insolensYH-8

Publisher Website
Google Scholar
Abstract
H. insolens YH-8, a thermophilic fungus isolated from manure and compost heaps, produced a significant amount of thermostable cellulases in cultures on wheat bran medium (50.degree. C, 4 days). The mold bran extract hydrolyzed Avicel, CMC [carboxymethylcellulase] and newsprint at 90%, 45% and 35%, respectively, to glucose. Then, Avicelase and CMCase [carboxymethylcellulase] were purified from the culture extract by adsorption onto Avicel, heat and acid treatment and consecutive column chromatographies to a homogeneous state on polyacrylamide gel disc electrophoresis. The purified cellulases, especially CMCase, were highly thermostable. The optimal temperature of both enzymes was 50.degree. C. Avicelase was stable after heating at 65.degree. C for 5 min and CMCase retained 45% of the original activity after heating at 95.degree. C for 5 min.

This publication has 0 references indexed in Scilit: