Distribution of collagenase and cell types in sterile ulceration of human corneal grafts

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Abstract
Extensive clinical and experimental evidence has suggested a role for corneal epithelium, keratocytes, and acute inflammatory cells in sterile corneal ulceration, although the precise role of these cell types in stromal matrix degradation has not been elucidated. We studied two corneal buttons and two whole corneas from 4 patients with corneal grafts and sterile stromal ulceration. Each cornea was divided into several sections and examined morphologically, while adjacent sections were placed in tissue culture and assayed for collagenase activity against type I collagen. In each case, collagenase activity was present in tissue from ulcerating and nonulcerating areas and, in most regions assayed, exceeded collagenase activity in normal control peripheral donor corneas. Collagenase levels were not always greatest in areas of most advanced ulceration. Although polymorphonuclear leukocytes were extensively present histologically in two cases, one case had a mixed inflammatory cell population at the site of ulceration, and another had only keratocytes (fibroblasts) present in the entire cornea. We conclude that regional differences in collagen degradation are due not only to the presence of collagenase, but also to cellular and humoral activators and inhibitors that determine collagenolytic activity.