DOUBLE-STRANDED RNA FROM TOBACCO LEAVES INFECTED WITH TMV
- 1 August 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 52 (2) , 401-408
- https://doi.org/10.1073/pnas.52.2.401
Abstract
Tobacco leaves systemically infected with TMV contain elements which are nuclease-resistant, alkali-labile, and are capable, after suitable dissociation and annealing, of conferring ribonuclease resistance upon P32-labeled TMV-RNA. The latter property is specific for TMV-RNA, and identifies the protective elements as RNA strands containing nucleotide sequences complementary to those in TMV-RNA, and which prior to dissociation were contained in double-stranded forms. The infected cells studied contain from 102 to 103 viral equivalents of doublestranded RNA, or one per 103 mature virus particles.Keywords
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