Effect of ACTH on Glucose Metabolism in Rat Adrenalin Vitro

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Abstract
Quartered rat adrenals were incubated in vitro with glucose-1-C14, glucose- 6-C14 and glucose-U-C14. The oxidation of glucose-l-Cu to CO2 was 1.3 times that of glucose-6-C14. ACTH increased the oxidation of all 3 labeled glucose molecules by 20%, and there was no change in the C-l:C-6 ratio. ACTH increased 4- to 6-fold the concentration of corticosterone in the medium. The glucose carbon incorporation into total lipid was not affected by ACTH, and the C-l:C-6 ratio remained unchanged. ACTH decreased glucose-C14 incorporation into glycogen, but no change in glycogen content was noted. In vivo administration of ACTH also failed to decrease the adrenal glycogen content. These observations do not support the importance of phosphorylase activation or of metabolism via the hexose monophosphate pathway in ACTH-stimulated steroidogenesis.