High-Resolution Typing of Toxoplasma gondii Using Microsatellite Loci
- 1 December 2001
- journal article
- Published by American Society of Parasitologists in Journal of Parasitology
- Vol. 87 (6) , 1472-1475
- https://doi.org/10.1645/0022-3395(2001)087[1472:hrtotg]2.0.co;2
Abstract
High-resolution typing of Toxoplasma gondii is essential to understand the effect of genetic differences among strains on the variation in disease manifestation and transmission patterns. Current typing methods discern 3 lineages with minimal within-lineage variation. Described here are 6 new variable loci. These loci, including a minisatellite and 5 microsatellites, were more polymorphic than allozymes, restriction fragment length polymorphisms, and sequence variation in introns. Most importantly, these loci revealed, for the first time, substantial within-lineage variation that was over 6-fold higher than that detected by other markers. Genotyping at these loci facilitates classification of isolates beyond the lineage level.Keywords
This publication has 11 references indexed in Scilit:
- A Single Amino Acid Substitution in Nonstructural Protein 3A Can Mediate Adaptation of Foot-and-Mouth Disease Virus to the Guinea PigJournal of Virology, 2001
- Complex mutations in a high proportion of microsatellite loci from the protozoan parasite Plasmodium falciparumMolecular Ecology, 2000
- STRAIN TYPING OFTOXOPLASMA GONDII:COMPARISON OF ANTIGEN-CODING AND HOUSEKEEPING GENESJournal of Parasitology, 2000
- Variation of Microsatellite Size Homoplasy Across Electromorphs, Loci, and Populations in Three Invertebrate SpeciesJournal of Molecular Evolution, 1998
- Low mutation rates of microsatellite loci in Drosophila melanogasterNature Genetics, 1997
- Biodiversity in Toxoplasma gondiiPublished by Springer Nature ,1996
- Specific DNA polymorphisms discriminate between virulence and non-virulence to mice in nine Toxoplasma gondii strainsMolecular and Biochemical Parasitology, 1995
- High resolution of human evolutionary trees with polymorphic microsatellitesNature, 1994
- Mutation of human short tandem repeatsHuman Molecular Genetics, 1993
- Virulent strains of Toxoplasma gondii comprise a single clonal lineageNature, 1992