Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer
Open Access
- 8 November 2010
- journal article
- Published by Springer Nature in Journal of Experimental & Clinical Cancer Research
- Vol. 29 (1) , 144
- https://doi.org/10.1186/1756-9966-29-144
Abstract
Real-time reverse transcription PCR (qRT-PCR) is frequently used for gene expression quantification due to its methodological reproducibility and sensitivity. The gene expression is quantified by normalization to one or more reference genes which are presumed stably expressed throughout a given experiment. The aim of this study was to validate a standardized experimental setup to identifying reference genes for normalization of qRT-PCR in the metastatic and non-metastatic colon cancer.Keywords
This publication has 39 references indexed in Scilit:
- Effects of decitabine on the expression of selected endogenous control genes in human breast cancer cellsMolecular and Cellular Probes, 2010
- PTCH1, a receptor of Hedgehog signaling pathway, is correlated with metastatic potential of colorectal cancerUpsala Journal of Medical Sciences, 2010
- ‘Desperate house genes’: the dramatic example of hypoxiaBritish Journal of Cancer, 2010
- Identification of endogenous control genes for normalisation of real-time quantitative PCR data in colorectal cancerBMC Molecular Biology, 2010
- MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experimentsBMC Molecular Biology, 2010
- Real-time RT-PCR normalisation; strategies and considerationsGenes & Immunity, 2005
- Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissuesMolecular and Cellular Probes, 2005
- Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genesLaboratory Investigation, 2005
- Validation of endogenous controls for gene expression analysis in microdissected human renal biopsiesKidney International, 2003
- A molecular signature of metastasis in primary solid tumorsNature Genetics, 2002