Pluripotent Haemopoietic Stem Cell Entry Into Dna Synthesis In Vitro After Stimulation By Ara-C Treatment In Vivo
- 1 July 1986
- journal article
- research article
- Published by Wiley in Cell Proliferation
- Vol. 19 (4) , 477-483
- https://doi.org/10.1111/j.1365-2184.1986.tb00746.x
Abstract
Non-cycling pluripotent bone marrow stem cells (CFU-S), taken 3 hrs after injection of 20 mg of Ara-C, have been shown to enter DNA synthesis at 1 to 3 hr after being cultured in .alpha.-medium. This phenomenon was observed when bone marrow was incubated as a plug, but not when incubated as a cell suspension in the present experimental conditions. These results suggest that a medullary structure is necessary in order to observe this effect and/or that accessory cells are destroyed during the process of single cell preparation.This publication has 12 references indexed in Scilit:
- Regulation of splenic CFU-S kinetics after cytosine arabinoside treatment in mice II. In vitro studies: Long-range modulators of the splenic CFU-SLeukemia Research, 1984
- INHIBITION OF CFU ENTRY INTO CYCLE BY A BONE-MARROW EXTRACT1977
- THYMUS DEPENDENCY OF BONE-MARROW STEM-CELL PROLIFERATION IN RESPONSE TO CERTAIN ANTIGENS1976
- STIMULATING FACTORS LIBERATED BY TREATED BONE-MARROW - INVITRO EFFECT ON CFU KINETICS1976
- Antigenic Stimulation of DNA Synthesis in the Haematopoietic Stem Cells of Axenic MiceNature, 1970
- Proliferative Activity of the Stem Cells in the Bone-marrow of Mice after Single and Multiple Irradiations (Total-or Partial-body Exposure)International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1970
- The Effect of Differing Demands for Blood Cell Production on DNA Synthesis by Hemopoietic Colony-Forming Cells of MiceBlood, 1965
- A Direct Measurement of the Radiation Sensitivity of Normal Mouse Bone Marrow CellsRadiation Research, 1961