A general method for isolation of high molecular weight DNA from eukaryotes
- 1 September 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (9) , 2303-2308
- https://doi.org/10.1093/nar/3.9.2303
Abstract
A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.Keywords
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