PLASMODIUM-CHABAUDI IN MICE - ADOPTIVE TRANSFER OF IMMUNITY WITH ENRICHED POPULATIONS OF SPLEEN LYMPHOCYTE-T AND LYMPHOCYTE-B

  • 1 January 1978
    • journal article
    • research article
    • Vol. 34  (5) , 821-830
Abstract
Thymectomized NIH and C57BL mice were more susceptible to P. chabaudi than controls, indicating a role for T [thymus-derived] cells in acquired immunity to the parasite. Enriched populations of T and B [bone marrow-derived] cells were prepared from the spleens of immune mice using nylon-wool columns, and were adoptively transferred to syngeneic non-irradiated mice or mice irradiated with 600 or 800 rad. Some immunity could usually be transferred with immune T, B and glass-wool (g.w.) filtered spleen cell populations. In the heavily irradiated mice, g.w. filtered immune spleen cells gave the best protection and the immune T cells the least. Preliminary attempts to show synergistic activity between immune T and B cells in irradiated mice were not successful.