INDUCTION OF MYELOID COLONY-STIMULATING ACTIVITY IN MURINE MONOCYTE TUMOR-CELL LINES BY MACROPHAGE ACTIVATORS AND IN A T-CELL LINE BY CONCANAVALIN-A

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (5) , 1414-1419
Abstract
Certain fibrosarcoma lines in culture and the [mouse] WEHI-3 myelomonocytic leukemia cell line secrete myeloid colony-stimulating activity (CSA) spontaneously. Other hematopoietic tumor cell lines were described in which CSA is produced constitutively or is inducible by immunostimulators. CSA production in macrophage and monocyte tumor lines is induced by lipopolysaccharide, zymosan, BCG, tuberculin purified protein-derivative preparation from mycobacteria, and dextran sulfate. Myeloma, mastocytoma and T[thymus-derived]-lymphoma lines do not produce CSA with or without these agents. The T-lymphocyte mitogen concanavalin A (but not phytohemagglutinin) induces CSA synthesis in 1 of 7 T-lymphomas tested. In most cases induction of CSA correlated with conditions of cell growth inhibition by the immunomodulators. However, other drugs that cause cytostasis or cytotoxicity do not lead to CSA production. Leukemic cells may retain sensitivity to normal regulatory events with resultant effects on host hematopoietic cell functions.