Peptides that block hepatitis B virus assembly: analysis by cryomicroscopy, mutagenesis and transfection
Open Access
- 1 December 1998
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 17 (23) , 6839-6845
- https://doi.org/10.1093/emboj/17.23.6839
Abstract
Peptides selected to bind to hepatitis B virus (HBV) core protein block interaction with the long viral surface antigen (L‐HBsAg) in vitro. High resolution electron cryomicroscopy showed that one such peptide binds at the tips of the spikes of the core protein shell. The peptides contain two basic residues; changing either of two acidic residues at the spike tip to an alanine greatly reduced the binding affinity. Transfection of hepatoma cells with a replication‐competent HBV plasmid gave significantly reduced production of virus in the presence of peptide, in a dose‐dependent manner. These experiments show that the interaction of L‐HBsAg with core particles is critical for HBV assembly, and give proof of principle for its disruption in vivo by small molecules.Keywords
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