Primary structure of the β‐subunit of bovine transducin deduced from the cDNA sequence
Open Access
- 28 October 1985
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 191 (2) , 235-240
- https://doi.org/10.1016/0014-5793(85)80015-6
Abstract
DNA complementary to the bovine retinal mRNA coding for the β‐subunit of transducin has been cloned by screening a cDNA library with oligodeoxyribonucleotide probes. Nucleotide sequence analysis of the cloned cDNA has revealed that this polypeptide consists of 340 amino acid residues (including the initiating methionine). Furthermore, cDNA hybridizable with a transducin β‐subunit cDNA probe has been cloned from a library derived from bovine brain poly(A)+ RNA. Comparison of the cloned cDNAs, in conjunction with blot hybridization analysis and S1 nuclease mapping of poly(A)+ RNA from bovine retina, brain and liver, suggests that the mRNAs coding for the β‐subunits of transducin and other guanine nucleotide binding proteins have the same protein‐coding sequence but partly different 5'‐noncoding sequences.Keywords
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