Antibody Response in Rainbow Trout (Salmo Gairdneri)

Open Access
Publisher Website
Google Scholar
Abstract
Summary: The successful application of the Jerne test to lymphoid cells from immunized rainbow trout was found to depend on the use of isologous C′ or C′ of closely related species. Of the mammalian, avian, amphibian, and fish C's tested, only sera of salmonid fish were active. Two types of plaques were seen, distinguishable by size—large plaques, 1 to 3 mm in diameter, and small plaques, 0.1 to 0.4 mm in diameter. Studies of the kinetics of plaque formation revealed that in the primary response, plaques first appeared 2 to 6 days after injection with antigen and peaked on about day 14. Plaque-forming cells were first detected in the anterior kidney. Gel filtration of normal trout and salmon sera on Sephadex G-200 yielded a pattern with three major protein peaks; complement activity necessary for plaque formation or for tube hemolysis was limited to the second peak. The third peak contained natural hemolytic activity which was manifested after prolonged incubation with SRBC's. It was demonstrated that this natural hemolytic activity of normal serum can take place in vivo.

This publication has 0 references indexed in Scilit: