Phospholipins. 1. Partition chromatography of egg-yolk phospholipins on cellulose

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Abstract
The separation reported by Bevan et al. (1951) of phospholipins containing ethanolamine and choline by partition chromatography on paper or on cellulose columns could not be confirmed. These methods effectively separated contaminating amino-acids which were carried by the phospholipins into clear ether or chloroform soln. Removal of these amino-acids by cellulose or by ion-exchange resins reduced the atomic N/P ratio of crude egg phospholipins from 1.12 to 1.00. Among the free amino-acids present in the crude phospholipins and in the fresh yolk, serine, glycine, threonine, alanine, lysine, arginine, proline, tyrosine, valine, leucine and phenylalanine were identified.

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