Partial Purification by Affinity Chromatography of Tyrosine Aminotransferase-Synthesizing Ribosomes from Hepatoma Tissue Culture Cells
- 1 May 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (5) , 1014-1018
- https://doi.org/10.1073/pnas.68.5.1014
Abstract
This report describes a cell-free protein-synthesizing system derived from hepatoma tissue culture cells in which the synthesis of tyrosine aminotransferase is enhanced in relation to total protein synthesis. The enhancement was achieved by using affinity chromatography to select a specific fraction of ribosomes in which the proportion of tyrosine aminotransferase synthesis is higher than it is in unfractionated ribosomes.Keywords
This publication has 22 references indexed in Scilit:
- Immunoglobulin biosynthesis: V. Light chain assemblyJournal of Molecular Biology, 1970
- Control of Specific Gene Expression in Higher OrganismsScience, 1969
- Preferential Synthesis of Ferritin and Albumin by Different Populations of Liver PolysomesScience, 1969
- On the synthesis of tyrosine transaminase in vitroBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell cultureJournal of Molecular Biology, 1968
- Single-step purification of avidin from egg white by affinity chromatography on biocytin-Sepharose columnsBiochemical and Biophysical Research Communications, 1968
- Biosynthesis of immunoglobulins: The separate classes of polyribosomes synthesizing heavy and light chainsJournal of Molecular Biology, 1967
- The effect of progressive immunization on polyribosomal size in lymphoid cells.Proceedings of the National Academy of Sciences, 1965
- INDUCED ENZYME FORMED ON BACTERIAL POLYRIBOSOMESProceedings of the National Academy of Sciences, 1964
- Studies on the ribosome-bound β-galactosidase of Escherichia coliJournal of Molecular Biology, 1963