Variable expression of O-antigen and the role of lipopolysaccharide as an adhesin inAeromonas sobria

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Abstract
On initial isolation of Aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767O) and translucent (3767T). Strain 3767O consistently produced lipopolysaccharide (LPS) core and O-antigen side chain, detectable by SDS-PAGE and by Western blotting with an O-antigen-specific monoclonal antibody. Strain 3767T produced LPS core but the amount of O-antigen was dependent on factors including growth medium and bacterial growth phase. Strain 3767T exhibited significantly lower levels of adhesion to HEp-2 cells than 3767O and this correlated with the level of LPS expression, with the greatest reduction (61%) at stationary phase when no LPS was detectable. The results implicate LPS as an adhesin for A. sobria 3767.