Differentiation of Anomeric & Positional Isomers of Acyclic & Cyclic Nucleosides by FAB Tandem Mass Spectrometry & NMR

Abstract
We have been interested in developing routine methods, using standard 1H and 13C NMR spectroscopy of assigning anomeric configuration for both acyclic and cyclic nucleosides of the types (1) & (2) respectively. In the latter class of compounds ve have also sought to determine the site of glycosidation (viz N1 or N3 with respect to the imidazole ring). We have extended this study using FAB tandem mass spectrometry1 to identify stereochemical differences.

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