Viewing of Complex Molecules of Ethidium Bromide and Plasmid DNA in Solution by Atomic Force Microscopy.
- 1 January 2001
- journal article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 49 (4) , 413-417
- https://doi.org/10.1248/cpb.49.413
Abstract
Tertiary structure changes in plasmid DNA, induced by ethidium bromide intercalation, have been observed in aqueous solutions by the use of an atomic force microscope. A relaxed closed circular pBR322 molecule became a positively supercoiled complex on the drug binding. The supercoiling always resulted in an interwound (or a plectonemic) form, but never a solenoidal (or a toroidal) form. A quantitative analysis of the compactness of such supercoiled complexes has been carried out.Keywords
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