Biosynthesis of RNA in antibody-producing tissues.
- 1 September 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 54 (3) , 975-982
- https://doi.org/10.1073/pnas.54.3.975
Abstract
RNA from spleen and lymph nodes of the rat has been characterized by a study of its sedimentation properties, kinetics of radioactive labeling, nucleotide composition, and template activity. Immunization results in an unusually rapid rate of synthesis of rRNA and rRNA precursors, which account for a large part of newly synthesized RNA of these tissues. The first newly made RNA to reach the microsomes is a fraction of low molecular weight with the characteristics of mRNA. There is also evidence for the existence in the cell of mRNA of higher mol wt, which probably corresponds to mRNA as it is synthesized in the nucleus. Newly made rRNA is transferred in the cytoplasm as subcellular particles; it appears very rapidly on the microsomes, 18S RNA preceding 30S RNA.Keywords
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