Processing of phosphoenolpyruvate carboxykinase (GTP) RNA in vivo.
- 1 July 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (13) , 4346-4350
- https://doi.org/10.1073/pnas.82.13.4346
Abstract
Nuclear RNA precursors to the mRNA for rat cytosolic phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) and nuclear RNA species smaller in size than the mature enzyme mRNA were identified by using hybridization probes specific for introns and exons from the gene. A number of processing intermediates, including an intact intron, were noted. Based on the size and distribution of the precursors observed, a pathway is proposed for phosphoenolpyruvate carboxykinase RNA processing that involves splicing of introns starting at the 5'' end of the RNA transcript and proceeding toward the 3'' end.Keywords
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