The cytotoxic action of Bax on yeast cells does not require mitochondrial ADP/ATP carrier but may be related to its import to the mitochondria
- 5 April 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 471 (1) , 113-118
- https://doi.org/10.1016/s0014-5793(00)01379-x
Abstract
The effect of the expression of murine Bax protein on growth and vitality was examined in Saccharomyces cerevisiae and compared with the effect of Bax in mutant cells lacking functional mitochondria. The cytotoxic effect of Bax on yeast does not require functional oxidative phosphorylation, respiration, or mitochondrial proteins (ADP/ATP carriers) implicated in the formation of the permeability transition pore in mammalian mitochondria. In the wild type S. cerevisiae the expression of Bax does not result in a severe effect on mitochondrial membrane potential and respiration. On the basis of Bax induced differences in the fluorescence of green fluorescent protein fused to mitochondrial proteins, it is proposed that Bax may interfere with one essential cellular process in yeast: the mitochondrial protein import pathway that is specific for the proteins of the mitochondrial carrier familyKeywords
This publication has 35 references indexed in Scilit:
- Bax and Adenine Nucleotide Translocator Cooperate in the Mitochondrial Control of ApoptosisScience, 1998
- The Import Route of ADP/ATP Carrier into Mitochondria Separates from the General Import Pathway of Cleavable Preproteins at thetrans Side of the Outer MembranePublished by Elsevier ,1998
- The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of ApoptosisScience, 1997
- Why are mitochondria involved in apoptosis? Permeability transition pores and apoptosis as selective mechanisms to eliminate superoxide‐producing mitochondria and cellFEBS Letters, 1996
- Mitochondria and programmed cell death: back to the futureFEBS Letters, 1996
- Inhibitors of permeability transition interfere with the disruption of the mitochondrial transmembrane potential during apoptosisFEBS Letters, 1996
- Mitochondrial control of nuclear apoptosis.The Journal of Experimental Medicine, 1996
- Role of mitochondria and C‐terminal membrane anchor of Bcl‐2 in Bax induced growth arrest and mortality in Saccharomyces cerevisiaeFEBS Letters, 1996
- The mitochondrial permeability transitionBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1995
- Obligatory requirement of intramitochondrial ATP for normal functionning of the eucaryotic cellBiochemical and Biophysical Research Communications, 1972