Malt Diastase And Ptyalin In Place of Saliva in The Identification of Glycogen
- 1 January 1947
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 22 (2) , 67-70
- https://doi.org/10.3109/10520294709108296
Abstract
Digestion in 1% U.S.P. malt diastase or in 1% ptyalin at 37 [degree]C for 1 hr. is an effective substitute for the salivary digestion test used by Bauer, by Bensley and others" for the identification of glycogen. Actually the test is not specific for glycogen, since diastase, ptyalin and amylopsin digest other polysaccharides than glycogen, notably starch. The enzymes should be dissolved in a buffered neutral saline soln. consisting of 8 g. NaCl, 1.3 g. Na2HPO4 and 0.8 g. NaH2PO4-H2O in 1 1. of water. Enzyme tests should be done on uncollodionized sections.Keywords
This publication has 2 references indexed in Scilit:
- Notes on TechnicStain Technology, 1942
- Comparison of Methods for Demonstrating Glycogen MicroscopicallyStain Technology, 1939