Fragmentation of Rat Serum Albumin by Cyanogen Bromide Cleavage and the Amino Acid Sequences of Four Fragments1

1 June 1976

journal article
research article
Published by Oxford University Press (OUP) in The Journal of Biochemistry

Vol. 79 (6) , 1183-1196
https://doi.org/10.1093/oxfordjournals.jbchem.a131173

Abstract

Rat serum albumin was cleaved into seven peptides by cyanogen bromide treatment followed by reduction and carboxymethylation. The amino acid composition and the N-terminus of each peptide and the amino acid sequences of four peptides were determined by conventional methods. An alignment of these peptides in the original protein was proposed in the light of the established sequences of bovine and human plasma albumin.

Keywords

This publication has 3 references indexed in Scilit:

[8] End-group analysis using dansyl chloride
Published by Elsevier ,1972
DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*
Annals of the New York Academy of Sciences, 1964
The Preparation and Enzymatic Hydrolysis of Reduced and S-Carboxymethylated Proteins
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