Uncoupling reagents and metabolism. 3. Effects of salicylate and 2:4-dinitrophenol on the incorporation of 14C from labelled pyruvate into the soluble intermediates of isolated rat tissues

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Abstract
The effects of 5mM-salicylate and 0.5 mM-2,4-dinitrophenol on the incorporation of C14 from [3-C14]pyruvate and L-[C14]glutamate into the soluble intermediates of chopped rat tissues has been studied. In the absence of the uncoupling reagents, the tissue preparations incorporated the isotope into substances known to be involved in the tricarboxylic acid cycle, in transamination and in synthetic reactions. Salicylate and dinitrophenol produced similar effects on the distribution of radioactivity in the soluble intermediates which appear to be explicable in terms of their uncoupling action on oxidative phosphorylation reactions. Salicylate, but not dinitrophenol, caused an increased incorporation of C14 from the [3-C14]pyruvate into glutamic acid in liver and kidney and also inhibited the activities Of rat-serum glutamic-pyruvic and glutamic-oxaloacetic transaminases. Salicylate and dinitrophenol had similar effects on the distribution of Cl4 from L-[C14]glutamate in chopped rat kidney. Some implications of these results are discussed.