Formation of a Soluble Stable Complex between Pristine C60‐Fullerene and a Native Blood Protein

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Abstract
Concern is growing about the potential impact of human exposure to carbonaceous nanomaterials (such as fullerenes) in the environment. A valid biological study of how native biomolecules interact with nanomaterials at the molecular level in physiological conditions requires the preservation of their physicochemical properties, yet most investigations rely on the use of modified fullerene conjugates or aggregates. We report the formation of a stable, water-soluble, well-defined complex between a single molecule of pristine C60-fullerene and a native protein, bovine serum albumin protein (BSA), with the normal three-dimensional structure of BSA preserved. The ability to produce a pristine C60-fullerene–BSA hybrid at a physiological pH range lays a solid foundation for studying carbonaceous materials, biodelivery systems, and transport mechanisms and for characterizing the potential effects of nanomaterials on wildlife and human health, both in vitro and in vivo.