Diluents for the Preservation of Ram Spermatozoa I. Diluents Used At 37°C and 5°C Containing Casein

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Abstract
Ram semen was diluted 40- to 50-fold in various synthetic diluents and the motility of the spermatozoa scored after incubation at 37[degree]C and after slow cooling to, and storage at, 5[deKree]C. Levels of potassium above 10 m-equiv. were deleterious at both temperatures, the addition of 5 m-equiv. calcium depressed motility at 37[degree]C but improved survival rates of spermatozoa stored at 5[degree]C. The inclusion of 6 m-equiv. magnesium in diluents had no detectable effect on motility scores. Replacement of the sodium chloride component (123 m[image]) of a diluent buffered with 20 m[image] phosphate buffer with an isomotic lactose solution showed that diluents with a content of this sugar ranging from 61 to 246 mM were superior at both 37 and 5[degree]C to that containing 123 m[image] sodium chloride and no lactose. Diluents containing 184 m[image] fructose, glucose, lactose, or sucrose and 31 mid sodium chloride were all better for the storage of ram spermatozoa at 5[degree]C than the diluent containing 123 m[image] sodium chloride. Motility scores of spermatozoa incubated at 37[degree]C were slightly, but significantly, lower in diluents containing 2.0% casein than in those with 0.5% casein. Scores at 5[degree]C showed that 2.0% casein gave much better survival than 0.5% casein.

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