The Biological Activities of Cytochrome c Hemeoctapeptide (CHP). (2). The Effects of CHP against the Tissue Damage caused by dl-Isoproterenol-induced Cardiac Infarct-like Angina

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Abstract
As described in the previous paper, an octapeptide fragment containing the heme moiety (CHP) and its re1ated compound, cytochrome c, showed a protective effect against the denaturation of cell and organelles due to oxygen deficiency. A new type of experimental hypoxia was employed to ascertain the activity of CHP against a local hypoxic effect in cardiac muscles. Racemic isoproterenol hydrochloride (i.p.) was given subcutaneously or intraperitoneally to male rats of the Wistar-Imamichi strain in doses of 3-20 mg/kg. A severe infarct-like necrosis was produced in the rats, especially at the left ventricle. In this experimental hypixia, the effect of CHP and its related compounds which were given intraperitonelly was examined by biochemical and histopathological methods. The decrease of enzyme levels such as GOT and GPT in cardiac tissue was less in the rats given CHP (1 mg/kg) than in those given saline as a control. Furthermore, it seems likely that the increment of GOT levels in serum at 5 hr after the i.p. administration was lower in the rats given CHP (1-10 mg/kg) and cytochrome (16 mg/kg) than in the controls. Hemin was not effective. Histopathological findings were similar to these biochemical observations. In addition to the results obtaihed in the two types of experimeta1 hypoxia such as hemic hypoxia and anoxic hypoxia, 2) the present results suggested that the physiological function of externally given cytochrome c may be attributed to the biological function of an effective intermediate of cytochrome c such as CHP.

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