Polysome Isolation by Sepharose Column Chromatography
- 1 January 1975
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 5 (3) , 247-255
- https://doi.org/10.1080/00327487508061575
Abstract
Polysomes from the mouse myeloma MOPC-21 were purified by gel filtration on Sepharose 6B, 4B and 2B columns. All three columns eliminated nearly all intracellular material smaller than 40 S subunits. In addition, passage through 4B and 2B columns substantially reduced the amount of subunits and monosomes in the preparations. Purified polysomes retained structural integrity when stored at -85°C for at least nine weeks.Keywords
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