Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel
Top Cited Papers
- 1 March 2001
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 19 (3) , 248-252
- https://doi.org/10.1038/85696
Abstract
RNA and DNA strands produce ionic current signatures when driven through an α-hemolysin channel by an applied voltage. Here we combine this nanopore detector with a support vector machine (SVM) to analyze DNA hairpin molecules on the millisecond time scale. Measurable properties include duplex stem length, base pair mismatches, and loop length. This nanopore instrument can discriminate between individual DNA hairpins that differ by one base pair or by one nucleotide.Keywords
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