A Simple Internally-Standardised Isocratic HPLC Assay for Vitamin B6in Human Serum
- 1 April 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 15 (5) , 897-914
- https://doi.org/10.1080/10826079208018843
Abstract
A simple method which is practical for use in a routine clinical chemistry laboratory, for assay of vitamin B6 vitamers in serum is presented. The procedure uses a C18 (ODS) analytical column and fluorimetric detection (325 nm excitation, 400 nm emission) after post column derivatisation with pH 7.5 phosphate buffer containing lg/1 sodium sulphite. The mobile phase is pH 2.5, 0.067 mol/1 KH2PO4 adjusted with concentrated orthophosphoric acid and containing 125 umol/1 sodium hexanesulphonate as an ion pairing agent. Pyridoxamine 5′-phosphate is suggested for use as an internal standard when assaying human serum.Keywords
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