Production of enterotoxins and toxic shock syndrome toxin by Staphylococcus aureus isolated from bovine mastitis in Brazil

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Abstract
The production of toxic shock syndrome toxin 1 (TSST‐1) and enterotoxins (SE) A, B, C and D by bovine mastitis isolates of Staphylococcus aureus was evaluated by immunodiffusion using the Optimum‐Sensitivity Plate method. S. aureus strains were isolated from bovine mastitis in 23 dairy herds in the state of Minas Gerais, Brazil, during 1994–9. Of 127 isolates, 83 (65·04%) produced one or several toxins, and among them production of SE was found in 54 (43·0%) isolates, of which 1138 (29·09%) secreted enterotoxin identified as type D. TSST‐1 was found in 5829 (45·723·0%) isolates.