A Rapid Chemical Means for Removing Labile Glycohemoglobin
- 1 August 1981
- journal article
- research article
- Published by American Diabetes Association in Diabetes
- Vol. 30 (8) , 700-701
- https://doi.org/10.2337/diab.30.8.700
Abstract
We have devised a rapid and convenient method of eliminating the contribution of labile glycosylated hemoglobin in the hemoglobin A, assay. The procedure requires a 30-min incubation of erythrocytes in 30 mM semicarbazide and 12 mM aniline (pH 5, 38°C). This chemical means of eliminating the labile fraction is as effective as a 14-h incubation in isotonic saline as measured with high-pressure liquid chromatography or electrophoresis methods. The removal of the labile glycosylated hemoglobin fraction preserves the assay as a reliable index of chronic metabolic control.Keywords
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