THE PERMEABILITY OF FROG SKIN TO UREA

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Abstract
Urea solution was placed on one side of frog skin, and the amount penetrating to the other side was determined by the Van Slyke-Cullen method. Tests were made on the permeability of frog skin to urea when the skins were bathed in isotonic solutions of varying proportions of CaCl2 and NaCl, with the control skins bathed in pure isotonic NaCl. CaCl2 decreased the permeability of the skin to urea, the greatest diminution being obtained in most cases with a solution consisting of 10% of a 1.1% CaCl2 solution plus 90% of a 0.7% NaCl solution. In many cases there was more penetration of urea into solutions of pure isotonic CaCl2 than into controls of NaCl.

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