Anti‐idiotype vaccine against Hodgkin's lymphoma: Induction of B‐ and T‐cell immunity across species barriers against cd30 antigen by murine monoclonal internal image antibodies

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Abstract
Murine monoclonal antibody HRS‐4 (Ab,), which defines the cell‐bound and soluble CD30 antigen associated with Hodgkin's lymphoma, was used to generate monoclonal anti‐idiotypic antibodies (Ab2) in syngeneic BALB/c mice. Murine monoclonal Ab2 14G9 and Ab2 9G10 directed against HRS‐4 were shown to be anti‐idiotypic Ab2β carrying the internal image of the CD30 antigen. These antibodies bound specifically to HRS‐4 and effectively inhibited binding of HRS‐4 to a CD30 antigen preparation at concentrations as low as 50 ng/ml. KLH‐coupled Ab2β 14G9 and 9G10 induced in BALB/c mice and New Zealand white rabbits a specific polyclonal humoral response against the 120 kDa band of the CD30 antigen. Moreover, BALB/c mice immunized i.p. with KLH‐coupled 14G9 and 9G10 exhibited a statistically significant (p < 0.01) delayed‐type hyper‐sensitivity reaction against CO30 expressing Hodgkin‐derived L540‐cells. We conclude from these data that Ab2β* 14G9 and 9G10, mimicking structures of the nominal CD30 antigen, are capable of inducing a CD30‐specific T‐cell‐ and B‐cell‐mediated immune response in mice and even across species barriers in rabbits. These CD30 anti‐id antibodies may hold promise for use as vaccines against CD30‐antigen‐expressing lymphomas.