HUMAN SOMATOTROPIN .53. SYNTHESIS AND BIOLOGICAL-ACTIVITY OF AMINO TERMINAL .54. RESIDUE FRAGMENT
- 1 January 1977
- journal article
- research article
- Vol. 10 (5) , 385-393
Abstract
The amino terminal 54 residue peptide fragment of human somatotropin, [Cys(Cam)53 [S-carbamidomethylcysteine]]-HGH[human growth hormone]-(1-54), was synthesized by the solid-phase method. The symmetrical anhydride and active ester coupling methods were used exclusively. The synthetic product was purified by gel filtration, isoelectric focusing and partition chromatography. It was homogeneous by 6 additional criteria. In complement fixation experiments the synthetic product was immunological active with antisera to HGH and [Cys(Cam)53]-HGH-(1-134). Antiserum raised against the synthetic product was immunologically active in the homologous assay and with HGH, [Cys,(Cam)53]-HGH-(1-134), and [Cys(Cam)53]-HGH-(15-125). The synthetic fragment exhibited 53% of the activity of [Cys(Cam)53]-HGH-(1-134) in the rat tibia assay.This publication has 8 references indexed in Scilit:
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