CHARACTERIZATION OF THE BINDING OF [3H]MUSCIMOL, A POTENT γ‐AMINOBUTYRIC ACID AGONIST, TO RAT BRAIN SYNAPTOSOMAL MEMBRANES USING A FILTRATION ASSAY
- 1 March 1979
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 32 (3) , 713-718
- https://doi.org/10.1111/j.1471-4159.1979.tb04553.x
Abstract
Abstract— The binding of [3H]muscimol, a potent GABA agonist, to crude synaptic membranes prepared from rat brain was studied using a filtration method to isolate membrane‐bound ligand. Specific binding was found to be saturable and occurred to two binding sites of Kd5 5 and 30 nm. Binding was Na+‐independent and enhanced by both freezing and Triton treatment. Regional and subcellular distribution studies and pharmacological characterization of specific [3H]muscimol binding are consistent with binding to the synaptic GABA receptor.This publication has 15 references indexed in Scilit:
- Muscimol binding in rat brain: Association with synaptic GABA receptorsBrain Research, 1978
- Use of 3H-muscimol for GABA receptor studiesNature, 1978
- GABA receptor binding with 3H-muscimol in calf cerebellumEuropean Journal of Pharmacology, 1978
- Neuropharmacology of Amino Acid Inhibitory TransmittersAnnual Review of Pharmacology and Toxicology, 1978
- GABA receptor binding with 3H (+) bicuculline–methiodide in rat CNSNature, 1977
- Stereospecificity and structure-activity requirements of GABA receptor binding in rat brainBrain Research, 1977
- Na+-independent binding of GABA to the triton X-100 treated synaptic membranes from cerebellum of rat brainLife Sciences, 1977
- Properties of γ-aminobutyric acid (GABA) receptor binding in rat brain synaptic membrane fractionsBrain Research, 1975
- Analysis of a Calcium Ion Binding System Composed of Two Different SitesBiophysical Journal, 1972
- REGIONAL STUDIES OF CATECHOLAMINES IN THE RAT BRAIN‐IJournal of Neurochemistry, 1966