Inhibition of nuclear DNA-dependent RNA polymerases from mouse ascites tumors and liver by glycerol
- 1 October 1977
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 55 (10) , 1117-1120
- https://doi.org/10.1139/o77-166
Abstract
Nuclear DNA dependent RNA polymerases were isolated from Ehrlich ascites carcinoma, TA3 ascites [mammary] adenocarcinoma and mouse liver and tested for inhibition by glycerol. The results confirm the finding of Smith and Duerksen that glycerol may inhibit nuclear RNA polymerase II, but because different grades of glycerol inhibited mouse liver RNA polymerase IIa to different extents, an inhibitory contaminant is present. RNA polymerases IIa and IIb from the 2 tumors and mouse liver were proportionately inhibited by A.C.S. [American Chemical Society] reagent grade glycerol at concentrations > 10%. RNA polymerase Ia from liver and the TA3 tumor was not inhibited by any concentration of glycerol tested (2-32.3%), but RNA polymerase Ia from Ehrlich carcinoma was inhibited by glycerol concentrations > 16%.This publication has 1 reference indexed in Scilit:
- Chemical modification and cross-linking of proteins by impurities in glycerolArchives of Biochemistry and Biophysics, 1976