Catabolite Repression of Cellulase Formation in Trichoderma viride*

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Abstract
Formation of cellulase [EC 3.2.1.4] in washed mycelia of Trichoderma viride induced by sophorose was competitively repressed by glucose. Pyruvate also showed a similar repressive effect. Glucose repressed the formation of constitutive enzymes such a β-glucosidase [EC 3.2.1.21], xylanase [EC 3.2.1.8] and amylase [EC 3.2.1.1] but had little or no effect on formation of protease or acid phosphatase [EC 3.1.3.2], which also seemed to be constitutive enzymes. The inhibition by glucose was compared with those by actinomycin D and puromycin. The former resembled that by puromycin, but differed considerably from that by actinomycin D in extent and in the period required for complete cessation of cellulase formation after its addition. Moreover, glucose was still inhibitory when added 60 miii after addition of actinomycin D by which time, further synthesis of mRNA specific for cellulase appeared to have ceased. These data suggest that catabolite repression of inductive cellulase formation in T. viride occurs mainly at the translational level of the cellulase protein.

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