HPLC assay of underivatized free amino acids with column switching and evaporative light‐scattering detection

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Abstract
In this communication the authors report the HPLC SEPARATION OF 20 amino acids without derivatization. Separation was achieved by column swithing using a C‐8 reversed‐phase column and a cationic ion‐exchange column. Detection of the underivatized amino acids was accomplished by means of an evaporative laser light‐scattering (ELS) detector. The influence of the temperature of the diffusion tube and nebuolzation gas pressure on detector response is reported. The relationship of peak areas vs. mass show a good log‐log linearity within a 1:10 range. Detechtion limit is in the order of 200 pmol for most amino acids under the conditions used.