OBSERVATIONS ON THE LIPOLYTIC ACTIVITY OF HUMAN SERUM AND PITUITARY FRACTIONS IN VITRO

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Abstract
SUMMARY Human sera and fractions derived from human pituitary tissue have been tested for lipolytic activity using intact epididymal fat pads of the rat and isolated cell suspensions prepared from rat fat pads and from human adipopose tissue. (1) Glycerol release was enhanced when human serum was incubated with epididymal fat pads and with isolated rat adipose tissue cells. No difference in this effect was noted between fasting serum and serum obtained 30 min. after glucose administration. (2) The three primary fractions of pituitary powder were assayed for lipolytic activity with rat cells, the one containing the gonadotrophins and thyroid-stimulating hormone (TSH) was highly potent while those containing human growth hormone (HGH) and corticotrophin respectively were inactive. The lipolytic activity was associated with TSH but not entirely due to TSH. (3) Human cells were prepared from adipose tissue from both sexes, from ages 4 to 78 and from subcutaneous, perirenal and intra-abdominal sites. (4) Compared with rat cells, human cells were less sensitive and less consistent in their pattern of response. Human cells reacted poorly or not at all to large doses of HGH while rat cells react to small doses.

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