Platelet Aggregation and the Ouabain-insensitive ATPase: Ecto-ATPase, Reflection of Membrane Integrity
Open Access
- 1 June 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 67 (6) , 528-532
- https://doi.org/10.1093/ajcp/67.6.528
Abstract
The ecto-ATPase activity of washed human platelets has been characterized by a higher-performance liquid chromatographic method. Mg++ was found to stimulate ecto-ATPase activity more strongly than Ca++. The combination of Mg++ and Ca++ at increasing concentrations caused diminishing activity levels. Elevated ecto-ATPase activity was also found in platelets incubated with prostaglandin E1 (PGE1) or potassium cyanide (KCN). It is proposed that the increase of ecto-ATPase activity is related to the extrusion or exposure of plasma membrane containing a ouabain-insensitive ATPase. The role of ecto-ATPase in platelet aggregation is discussed in light of these findings.Keywords
This publication has 1 reference indexed in Scilit:
- Platelet agglutination and deagglutination with a sulfhydryl inhibitor, methyl mercuric nitrate: Relationships to platelet ATPaseExperimental and Molecular Pathology, 1965